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LB膜分析儀

更新時(shí)間：2025-10-22

點(diǎn)擊次數(shù)：3167

　　LB膜分析儀是測(cè)定極性有機(jī)物(兩親分子)物理化學(xué)特性的精密測(cè)量?jī)x器?？梢詣?dòng)態(tài)地研究各種有極性有機(jī)物質(zhì)(蛋白質(zhì)，脂質(zhì)，高聚物等)的單分子層表面膜，記錄膜的分子表面積( A)與表面張力( r)或表面壓力(π)之間的函數(shù)關(guān)系?？捎糜趯?duì)相分離、相變、微區(qū)形成、成核過(guò)程和在單層膜組裝密度精確控制中酶的作用的實(shí)時(shí)熒光成像。也可被當(dāng)作一個(gè)張力計(jì)使用，監(jiān)控在較低的亞相體積下所期望得到組成的脂質(zhì)單層和藥物/肽等物質(zhì)的相互作用。

　　LB膜分析儀的工作原理：

　　位于氣-液或液-液界面處不可溶的功能性分子、納米顆粒、納米線或微粒所形成的單分子層可定義為L(zhǎng)angmuir膜。這些分子能夠在界面處自由移動(dòng)，具有較強(qiáng)的流動(dòng)性，易于控制其堆積密度，研究單分子層的行為。將材料沉積在淺池中的水亞相上，可以得到Langmuir膜。在滑障的作用下，單分子層可以被壓縮。表面壓力即堆積密度可以通過(guò)Langmuir膜分析儀的壓力傳感器進(jìn)行控制。

　　在進(jìn)行的等溫壓縮測(cè)試時(shí)，單分子層先從二維的氣相(G)轉(zhuǎn)變到液相(L)*后形成有序的固相(S)。在氣相中，分子間的相互作用力比較弱；當(dāng)表面積減小，分子間的堆積更為緊密，并開(kāi)始發(fā)生相互作用；在固相時(shí)，分子的堆積是有序的，導(dǎo)致表面壓迅速增大。當(dāng)表面壓達(dá)到*大值即塌縮點(diǎn)后，單分子層的堆積不再可控。單分子層膜狀態(tài)受表面壓力增加的影響。LB膜沉積過(guò)程是將樣品從單分子層中垂直拉出，通過(guò)反復(fù)沉積技術(shù)可制備多層LB膜，親水性及疏水性樣品均可在液相或氣相中沉積為單分子層。

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